Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Luteolin Inhibits the Biofilm Formation and Cytotoxicity of Methicillin-Resistant Staphylococcus aureus via Decreasing Bacterial Toxin Synthesis

doi: 10.1155/2022/4476339

Figure Lengend Snippet: Luteolin decreased the cytotoxicity of MRSA, and downregulated the expression of the hemolysin and hlaA genes in MRSA (a) Human bronchial epithelial cells were infected with luteolin-treated MRSA N315, and then the viability of human bronchial epithelial cells was measured using the CCK-8 assay. (b) Expressions of α -hemolysin and δ -hemolysin in luteolin-treated MRSA N315 were evaluated by western blot. (c) Level of hlaA in luteolin-treated MRSA N315 was detected using RT-PCR, and 16S was used as an endogenous control. ∗∗∗ P < 0.001, vs. Blank; ## P < 0.01, ### P < 0.001 vs. Control. (MRSA: methicillin-resistant Staphylococcus aureus , CCK-8: Cell Counting Kit-8, 16S: 16S rRNA, a -hemolysin: alpha hemolysin, d -hemolysin: delta hemolysin, RT-PCR: Real-Time PCR).

Article Snippet: Human bronchial epithelial cells (HBEs; CL-0346) were purchased from Procell (Wuhan, China) and grown in the specific cell medium (CM-0346, Procell, Wuhan, China).

Techniques: Expressing, Infection, CCK-8 Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Control, Cell Counting, Real-time Polymerase Chain Reaction

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long non-coding RNA NKILA inhibits proliferation and migration of lung cancer via IL-11/STAT3 signaling

doi:

Figure Lengend Snippet: The expression of NKILA was higher in normal human bronchial epithelial cell line. For (A) and (B), the expression level of lncRNA-NKILA was analyzed by Q-PCR. The expression of NKILA was analyzed in NSCLC tissues (C), Kaplan-Meier analyses of the correlations between lncRNA-NKILA expression level and survival (D). Values are means ± SEM for n = 7-8. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control.

Article Snippet: Cell culture A normal human bronchial epithelial cell line (16HBE) and NSCLC adenocarcinoma cell lines (A549, NCI-H1975) were purchased from the Biopike Biological company.

Techniques: Expressing, Control

Journal: International Journal of Clinical and Experimental Pathology

Article Title: CXCR4 inhibitor attenuates allergen-induced lung inflammation by down-regulating MMP-9 and ERK1/2

doi:

Figure Lengend Snippet: Results for immunostaining of CXCR4 in bronchial epithelial cells. CXCR4 in 16HBE cells were first probed with a rabbit derived mAb and then stained a green fluorescent labeled anti-rabbit IgG (green). Nuclei were stained in red by PI (original magnification × 400).

Article Snippet: Cell culture and treatment The human bronchial epithelial cell line (16HBE) was obtained from Fuxiang Biotechnology Co. Ltd (Shanghai, China).

Techniques: Immunostaining, Derivative Assay, Staining, Labeling

Journal: International Journal of Clinical and Experimental Pathology

Article Title: CXCR4 inhibitor attenuates allergen-induced lung inflammation by down-regulating MMP-9 and ERK1/2

doi:

Figure Lengend Snippet: CXCL12/CXCR4 synergizes with IL-13 to enhance epithelial MMP-9 expression. A. CXCL12 time-dependently induced epithelial cells expression of MMP-9. 16HBE cells were cultured in serum-free medium at 37°C for 24 h and then stimulated with CXCL12 (200 ng/ml) as indicated times. B. Gelatin zymographic results for conditioned media collected from CXCL12 treated 16HBE cells. C. Western blot analysis of epithelial MMP-9 expression after CXCL12 and/or IL-13 stimulation. 16HBE cells were treated for 24 h with 10 ng/ml IL-13, 200 ng/ml CXCL12, 10 ng/ml IL-13 and 200 ng/ml CXCL12, CXCL12 and saline, CXCL12 and AMD3100, respectively. The cells were then harvested for Western blot analysis of MMP-9 expression. D. A bar graphic figure showing the results of 5 independent experiments conducted. *, P < 0.05 as compared with Control group; #, P < 0.05 as compared with CXCL12 group.

Article Snippet: Cell culture and treatment The human bronchial epithelial cell line (16HBE) was obtained from Fuxiang Biotechnology Co. Ltd (Shanghai, China).

Techniques: Expressing, Cell Culture, Western Blot, Saline, Control

Journal: International Journal of Clinical and Experimental Pathology

Article Title: CXCR4 inhibitor attenuates allergen-induced lung inflammation by down-regulating MMP-9 and ERK1/2

doi:

Figure Lengend Snippet: CXCL12/CXCR4 signaling induces ERK1/2 expression and activation. 16HBE cells were incubated with 200 ng/ml CXCL12 as the indicated time and then subjected to Western blot analysis of ERK1/2 expression and activation. A. CXCL12 potently increased total ERK1/2 protein levels and pERK1/2 levels. The results are a representative of 3 independent experiments conducted. B. Blockade of ERK1/2 signaling by PD98059 abolished the stimulatory effect of CXCL12 on epithelial MMP-9 expression. 16HBE cells were pretreated with PD98059 (50 μM) for 30 min before CXCL12 stimulation. Addition of PD98059 completely diminished CXCL12 induced MMP-9 expression in 16HBE cells.

Article Snippet: Cell culture and treatment The human bronchial epithelial cell line (16HBE) was obtained from Fuxiang Biotechnology Co. Ltd (Shanghai, China).

Techniques: Expressing, Activation Assay, Incubation, Western Blot

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Long Non-coding RNA snaR Promotes Proliferation in EGFR Wild Type Non-Small Cell Lung Cancer Cells

doi: 10.22088/IJMCM.BUMS.10.4.258

Figure Lengend Snippet: snaR expression in normal human bronchial epithelial cells 16HBE and NSCLC cell lines. Data are presented as mean ± SD (*P < 0.05, **P < 0.01)

Article Snippet: C ell lines and culture conditions NSCLC cell lines including two carrying wild type EGFR (A549, SPC-A1) and two carrying mutations in EGFR (PC9 and H1975: PC9 cells carry a Glu746-Ala750 deletion mutation in exon 19 of the EGFR gene, and H1975 cells carry two-point mutations, T790M and L858R, in exons 20 and 21, respectively), and a normal human bronchial epithelial cell line (16HBE) were purchased from the Cell Bank of the Pasteur Institute of Iran (Tehran, Iran).

Techniques: Expressing